Colloidal Gold Immunoassay Strip Instructions of Xanthomonas oryzae pv.oryzicola (Xoc)

Ⅰ. Testing Principle:

The colloidal gold immunotest strip for the detection of Xanthomonas oryzae pv.oryzicola is a qualitative test strip. The paper strip is based on the formation of double antibody sandwich immune complex on the detection line of nitrocellulosic membrane to detect Xanthomonas oryzae pv.oryzicola.

Ⅱ. Range of Application：

This product is used for rapid detection of Xanthomonas oryzae pv.oryzicola in rice leaf or seed samples.

Ⅲ. Packing Specification:

  10pcs/box   

Ⅳ. Operating Steps:

（1）    Sample Preparation:

Rice leaves: Take about 0.1 g of rice leaves with obvious symptoms and put them into a mortar. Pour 1 ml of the extract from the sample handling tube into the mortar containing the leaves. Grind the homogenized sample with a pestle for 3 minutes.

Seed samples: Randomly take 10 seeds from each sample and put them into a sample processing tube containing 1 mL of extraction solution, and shake them at room temperature for 20-30 min; Or 10 seeds were cut along the side, the rice part was peeled, and the remaining seed shells were collected and placed into a sample processing tube containing 1 mL of extraction solution, and shook at room temperature for 20-30 min (recommended).

（2）    Sample Test:

Remove the strip from the aluminum foil bag and lay it flat face up. Use a plastic straw to absorb the leaf homogenate or seed extract. Add 3-4 drops (about 100µL) into the sampling hole (S) of the strip and place it for 5-10 minutes to judge the result.

Ⅴ. Result Interpretation:

Negative (-) : Line C shows color, line T does not show color, and is judged as negative.

Positive (+) : C line color, T line also color, regardless of the color of the T line is judged to be positive.

Invalid: No C line appears, regardless of whether the T line is colored or not, indicating an incorrect operation process or the detection strip has failed. In this case, the instructions should be read carefully again and retested with a new test strip.

Ⅵ. Notes:

(1) Perform the test according to the procedure. Do not touch the color development area of the test strip during operation.

(2) This product is a disposable product, do not reuse.

(3) Do not use tap water, pure water, distilled water and normal saline as a negative control or sample extraction solution.

(4) Do not mix plastic straws from different samples.

(5) After taking out the test strip from the original packaging aluminum foil bag, it should be used within 1 hour.

(6) Place the test strip on a flat table for testing, and add 3-4 drops of sample extraction solution vertically.

(7) Because the bacteria will precipitate at rest, the sample extract is slightly mixed before the plastic straw is sucked.

(8) Due to differences between samples, T, C line color glossiness will be different, which is a normal phenomenon.

(9) If the ambient temperature is too low in the experiment, the test strip will be abnormal. Please keep the ambient temperature at 4-37℃.

VII. Storage Condition and Validity:

Storage conditions: Store test strips in a cool dry environment away from light at 4-30℃. Do not freeze.

Validity: The product is valid for 24 months. The date of manufacture is on the box.